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Study on antioxidant and anti-cancer effects of an extracted mixture of papaya leaves and Panax pseudoginseng on hepatocellular carcinoma (HepG2) and lung cancer (LU).

Description: Liver cancer and lung cancer are increasingly common and are one of the causes of death for humans. Free radicals accumulate in the body, causing damage to the body, causing serious diseases, cell aging and causing human death through cancers such as liver and lung cancer. In addition, antibiotic-resistant microorganisms appear more and more and are also the cause of death, especially for children. That situation requires scientists to research, find new active ingredients to fight dangerous diseases and protect the body. With the tropical climate, the plant species in Vietnam are very diverse and plentiful, many of which contain the above biological compounds. Many scientist are interested in using these plants in researches to find active ingredients that enhance health and prevent diseases, especially liver and lung cancer. Among the precious medicinal herbs, papaya leaves and Panax pseudoginseng are the species that scientists like to use in researches The papaya leaves and Panax

OUR MATERIALS, CHEMICALS 1. n-Hexane, ethyl acetate, butanol, some other chemicals and solvents are commercial products of China. 2. Papaya leaves were collected in Bao Thang District, and the Panax pseudoginseng were collected at Si Ma Cai, Lao Cai Province. METHODS 1. Extraction of the study sample by fractional extraction with increasing polarization of the selected solvents (n-hexane, ethyl acetate and ethanol) for extraction. 2. Isolation of compounds by appropriate methods (fractional crystallization, column chromatography (CC), HPLC, etc.). 3. The use of modern spectroscopic physical methods (ultraviolet, infrared, mass spectroscopy, 1D and 2D nuclear magnetic resonance spectroscopy techniques) in structural analysis and identification of isolated compounds TECHNIQUES: Infrared Spectroscopy (IR) , Energy-dispersive X-ray spectroscopy (EDX) , Dynamic light scattering (DLS), Scanning electron microscope (SEM), High-performance liquid chromatography (HPLC), Ultraviolet-visible spectra (UV-Vis) EXTRACTION AND FRACTIONATION STEPS: Firstly , we extracted Papaya leaves and Panax pseudoginseng and dry those at 500*C. Then , we crush and soak this material in methanol and filter into solution . With the remaning residue , we continue crushung and soaking in methanol for 3 times . With the solution from filtration , we evaporate in solvent which results in condensed solution . After that , we fractionate previous condensed solution with n- hexane Solvent evaporation , Ethyl acetate Solvent evaporation , and Butanol Solvent evaporation RESULTS: The n-hexane fractions from papaya leaves and Panax pseudoginseng both exhibit antioxidant, antibacterial and inhibitory activity on cancer cells. In particular, the antibacterial activity was strongly demonstrated in the papaya leaves while the inhibitory activity of cancer cells was strongly demonstrated in Panax pseudoginseng. The combination of n-Hexane extracts from papaya leaves and Panax pseudoginseng at the rate of 1/1 has significantly increased antioxidant, antibacterial and anti-cancer activities compared to the extracts of each material. Antioxidant activity of papaya leaves : IC50 value of the papaya extracts was relatively low, meaning that the antioxidant activity of the papaya extracts was quite high. Some studies on other species of the genus also show that the extract has quite strong antioxidant activity. Research of Lin et al (2009) on species has recorded very positive antioxidant activity from prenylflavonoid active substance in extracts. Similarly, research on species of Singhatong et al. (2010) also showed that antioxidant activity was quite strong from leaf extract. Antibacterial activity of papaya leaves : The lower the IC number, the higher the antimicrobial activity. Results showed that, antimicrobial activity gram (+) was higher than antimicrobial activity gram (-) in all segments. In this study, the antimicrobial activity of papaya leaf extract was quite wide with both gram (+) and gram (-) groups and mainly due to substances of the nHexan fraction expressed, antimicrobial activity. Gram (+) bacteria were higher than gram (-) antimicrobial activity. This result also demonstrates the anti-inflammatory effect of some species in the genus Papaya has been used in traditional medicine; contributes to the orientation for the use and exploitation of papaya leaves as a plant antibiotic to cure diseases caused by gram bacteria, especially gram (+) bacteria Anti-cancer activity of papaya leaves : the extracts from papaya leaf extract all had cytotoxic activity in both experimental cell lines of LU and HepG2 Antioxidant activity of MIXTURE OF PAPAYA LEAF EXTRACTS AND PANAX PSEUDOGINSENG EXTRACTS : the combination of n-Hexane solutes from papaya leaves and Panax pseudoginseng at the rate of 1/1 has improved antioxidant resistance compared to substances in the same fractions


Innovator(s): Quach Huyen Thuong, Trinh Duc Vinh, Nguyen Ngoc Mai Linh

Category: Biotechnology

Country: Vietnam

Gold Award