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α-GLUCOSIDASE INHIBITORY AND PHYTOCHEMICAL INVESTIGATION OF LUDWIGIA OCTOVALVIS COLLECTED IN VIETNAM

Description: The efficient and systematic α-glucosidase inhibition-guided fractionation of the n-hexane and ethyl acetate extracts of Ludwigia octovalvis was conducted and led to separation and purification of two flavonoids, luteolin (1), and orientin (2) using variously chromatographic methods. For the first time, compound 2 was recognized from this species.

The efficient and systematic α-glucosidase inhibition-guided fractionation of the nhexane and ethyl acetate extracts of Ludwigia octovalvis was conducted and led to separation and purification of two flavonoids, luteolin (1), and orientin (2) using variously chromatographic methods. Their chemical structures were characterized by HR-ESI-MS, NMR experiments, and comparison with previous literature. For the first time, compound 2 was recognized from this species. Previous studies have demonstrated that both luteolin and orientin compounds have very strong α-glucosidase inhibitory activity compared with acarbose positive control with IC50 of 13.07 and 23.30 μM, respectively[16]. Based on data of 1H-NMR, 13C-NMR, and comparison with previously published data: the structure of LOE.01 was evidenced as 5,7,3’,4’-tetrahydroxyflavone (luteolin) [14] ; the structure of LOE.02 was evinced as luteolin 8-C-β-D-glucopyranoside (orientin)[15] . In summary, the systematic study on the chemical composition of the whole plants of Ludwigia octovalvis collected in Binh Chanh District, Ho Chi Minh City was described from sample collection, sample identification, purification, characterization of isolated compounds. Phytochemical investigation of the active extracts notification two flavonoids that both have very strong inhibitory activity on the enzyme α-glucosidase to clarify and improve the value of using local spinach and suggesting more in-depth studies on the composition of active ingredients as well as the mechanism of action of this species in the reduction and treatment of type-II diabetes.

Organisation: Research Institute of Creative Education (RICE)

Innovator(s): Huynh Kim Ngan, Ho Dac Huy

Category: Young inventors

Country: Vietnam

Gold Award