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Extraction of amylase enzyme from fruit waste used as a catalyst to enhance carbohydrate-waste based bioethanol production

Description: With the increasing amount of waste production, along with the increase in energy demand, there is great opportunity for extensive research on solutions to address these issues. Fruit wastes from Indonesian fruits like Mangosteen, Jackfruit, and Rambutan were experimented on and further processed into fruit juices.Using the centrifuge method, enzymes were extracted and tested upon an agar solution, resulting in a positive result of amylase enzyme within all of these different fruit wastes. Testing with the DNS method where glucose was added and broken down by these enzymes, the solution was tested in a spectrometer, where the absorbency of light of each solution was calculated.

The results showed that Jackfruit had the highest absorbency of light, which means that the glucose concentration was furthest broken down in the solution, proposing that the enzyme reaction was the strongest in this solution. With this data, the enzyme taken from Jackfruit waste was used as a catalyst for the next step, which was to convert food waste into bioethanol. Rice waste was heated up and used as a substrate in a solution where Sacharomyces cereviceae and the enzyme extracted from the jackfruit waste was added. After 7 days of fermenting the solution, the solution was then put through the distillation process for 3 hours, where 1.563% of ethanol was attained from 300mL of rice solution. With the amount of excess waste in Indonesia, there is plausibility and potential in producing a much higher concentration and amount of bioethanol. This experiment explores utilizing a limitation and converting it into a benefit. Further improvements are currently being done to further develop the applicability of this method to address both the problem of waste management and energy demand.

Organisation: Sekolah Pelita Harapan Sentul City

Innovator(s): Amrita Wikara, Maria Dyandra Dagna Sucianto

Category: Environment

Country: Indonesia

Gold Award