Innovative method of differentiating adipose tissue-derived cells into bone cells

Description: This innovation allows for very effective priming of human Adipose tissue Stem Cells (ASCs) into bone cells. The priming can be achieved by 3-4 days of ASCs culture on specific cell growth-supporting composites and thanks to defined culture media supplements and controlled flow of culture media.

This innovation provides a new method of differentiating Adipose tissue Stem Cells (ASCs) into bone cells. Human ASCs priming to osteogenic phenotype can be achieved as soon as by 3-4 culture days thanks to the application of specific cell growth-supporting composites, defined culture media supplements and controlled flow of culture media. The new method is based on: 1) seeding ASCs on PLGA-bioactive glass material sheets of defined chemical compositions; 2) stimulating ASCs with qualitatively and quantitatively defined media supplements; and 3) subjecting ASCs cultures at defined culture intervals to controlled media flow that induces a defined sheer stress. ASCs primed to bone cells by this method can be then transferred to any other delivery vehicle without losing their osteogenic potential or delivered alone, e.g. in suspension. The new method offers great feasibility as for handling and timing in any biological/clinical laboratory.

Organisation: Jagiellonian University

Innovator(s): Osyczka Anna-Maria, Truchan Karolina, Cholewa-Kowalska Katarzyna (AGH), Zagrajczuk Barbara (AGH)

Category: Medicine, Biotechnology and Medical Devices

Country: Poland